EPA Science Inventory

COMPARISON OF AN IN VIVO FISH VTG ASSAY WITH YES AND E-SCREEN

Citation:

Folmar, L C., M J. Hemmer, N D. Denslow, K. J. Kroll, A. Cheek, G. Grau, H. Richman, AND H. Meredith. COMPARISON OF AN IN VIVO FISH VTG ASSAY WITH YES AND E-SCREEN. Presented at Annual Meeting of the Society for Integrative and Comparative Biology, Denver, CO, 6-10 January 1999.

Description:

This study compares the efficacy of two in vitro, estrogen-sensitive bioassays to rank the "relative estrogenicity" of five natural, pharmaceutical and xenoestrogens with a newly developed in vivo bioassay. The E-SCREEN (MCF-7 tumor cells) and YES (Yeast Estrogen Screen) assays were used to rank estradiol-17B, ethynylestradiol, diethylstilbestrol, methoxychlor and nonyl phenol for their threshold activation and EC50 concentrations. Those rankings were compared with threshold and EC50 effects concentrations to induce vitellogenin synthesis in male sheepshead minnows exposed to those chemicals in a flow-through aqueous exposure. To eliminate any variability due to purity or compounding differences, test chemicals for all assays were from the same source.

Record Details:

Record Type: DOCUMENT (PRESENTATION/ABSTRACT)
Start Date: 01/06/1999
Completion Date: 01/06/1999
Record Last Revised: 12/22/2005
Record Created: 12/07/2004
Record Released: 12/07/2004
Record ID: 92803

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LAB

GULF ECOLOGY DIVISION