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HYBRIDIZATION STUDY BETWEEN GENETICALLY MODIFIED BRASSICA NAPUS AND NON-GENETICALLY MODIFIED B. NAPUS AND B. RAPA
Bautista, N. S., T Shiroyama, AND L S. Watrud. HYBRIDIZATION STUDY BETWEEN GENETICALLY MODIFIED BRASSICA NAPUS AND NON-GENETICALLY MODIFIED B. NAPUS AND B. RAPA. Presented at Joint Meeting of the 14th Crucifer Genetics Workshop and the 4th ISHS Sympos. on Brassicas, Daijon, Korea, Oct. 24-28, 2004.
Gene exchange between cultivated crops and wild species has gained significance in recent years because of concerns regarding the potential for gene flow between genetically modified (GM) crops and their domesticated and wild relatives. As part of our ecological effects of gene flow study in Brassica, we have made reciprocal crosses of GM canola, B. napus (cv. Raider) with non-GM Brassica species, i.e. B. napus (cv. Sponsor) and B. rapa. We have obtained F1 hybrids for the following crosses: GM B. napus (cv. Raider) x non-GM B. napus (cv. Sponsor); GM B. napus (cv. Raider) x B. rapa; GM B. napus (cv. Raider) x B. napus. Preliminary results have shown that a higher percentage of viable F1 seeds are produced when the GM parent was used as the pollen donor as compared to the reciprocal cross. This will be further evaluated in the succeeding F2 and backcross populations. Earlier germination also was observed in F1 progeny when the male parent was cv. Raider. Whether these observations are cultivar-specific or related to the presence of a GM gene needs to be further evaluated. We are in the process of developing the mapping populations needed for quantitative trait loci (QTL) analysis of traits related to ecological fitness. Once the mapping populations are obtained, genotyping will be done using molecular markers such as micro satellites to determine the location of the genes responsible for ecological fitness traits related to seed production, viability and germination.