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PFOA INDUCES DYSMORPHOGENESIS IN MOUSE WHOLE EMBRYO CULTURE
Citation:
Blanton, M R., J. M. Padowski, E S. Hunter III, J M. Rogers, AND C S. Lau. PFOA INDUCES DYSMORPHOGENESIS IN MOUSE WHOLE EMBRYO CULTURE. Presented at Society of Toxicology, New Orleans, LA, March 6-10, 2005.
Description:
PFOA Induces Dysmorphogenesis In Mouse Whole Embryo Culture.
MR Blanton1, JM Padowski2, ES Hunter1, JM Rogers1, and C Lau1. 1Reproductive Toxicology Division, NHEERL, ORD, US EPA, RTP, NC, USA. 2Curriculum in Toxicology, UNC, Chapel Hill, NC, USA
Perfluorooctanoate (PFOA) is a perfluoroalkyl acid (PFAA) found in numerous industrial and consumer products. Many PFAAs persist in the environment and are found in humans and animal tissues throughout the world. PFOA is a developmental toxicant in vivo producing embryonic, fetal and postnatal lethality, and altered fetal and neonatal growth. In order to determine if direct exposure of conceptuses to PFOA disrupts development, CD-1 mouse conceptuses (3-6 somite stage, GD8) were exposed to PFOA in whole embryo culture. At the end of a 24H culture period embryonic morphology was assessed and scored using a scoring system developed by our laboratory. Scores ranged from 0, for morphologically normal, to a maximum of 58 for severely affected embryos. In control medium, 82% of embryos (18/22) grew normally and the mean score was 0.4?0.2. Exposure to 0.1 or 0.2mg/ml PFOA did not alter development (4/4 and 11/17, normal embryos respectively). However, dysmorphogenesis was induced by PFOA at 0.4 (82%), 0.6 (92%), 0.75(100%) and 1.0 (100%) mg/ml. Exposure to 1.25mg/ml produced 100% embryolethality. Prosencephalic and pharyngeal arch hypoplasia and abnormal heart outflow tract development were induced by >0.4mg/ml PFOA. Embryonic scores were increased at PFOA concentrations >0.4mg/ml and were 0.0?0.0(0.1), 1.7?0.7(0.2), 11.3?2.2(0.4), 11.3?2.0(0.6), 23.5?1.5(0.75) and 31.1?3.9(1.0). The benchmark concentration for a 5% increase in dysmorphic embryos by PFOA was 0.04?0.01mg/ml. These studies demonstrate that a direct exposure to PFOA for 24H disrupts development and induces embryolethality. This abstract does not represent EPA policy.