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DEVELOPMENT OF DIOXIN TOXICITY EVALUATION METHOD IN HUMAN MILK BY ENZYME-LINKED IMMUNOSORBENT ASSAY-ASSAY VALIDATION FOR HUMAN MILK. (R825433)
Citation:
Sugawara, Y., K. Saito, M. Ogawa, S. Kobayashi, G. Shan, J. R. Sanborn, B. D. Hammock, H. Nakazawa, AND Y. Matsuki. DEVELOPMENT OF DIOXIN TOXICITY EVALUATION METHOD IN HUMAN MILK BY ENZYME-LINKED IMMUNOSORBENT ASSAY-ASSAY VALIDATION FOR HUMAN MILK. (R825433). CHEMOSPHERE 46(9-10):1471-1476.
Description:
In this study, the development of a toxicity evaluation method for dioxins in human milk by enzyme-linked immunosorbent assay (ELISA) was reported. A total of 17 human milk samples were tested by ELISA and by gas chromatography/mass spectrometry (GC/MS) to assess whether the ELISA performed on samples obtained from primiparas could be considered as reliable enough for identifying a dioxins contamination in human milk. The concept of toxicity equivalent quantity (TEQ) screening was validated by comparing TEQ values for a set of human milk samples to the ELISA responses predicted for those samples. A fairly good correlation (r=0.920) between immunoassay and GC/MS was achieved for human milk. This ELISA should be useful for biological samples monitoring.
Author Keywords: TCDD; Immunoassay; Validation; PCDDs; PCDFs; GC/MS