Science Inventory

A RAPID DNA EXTRACTION METHOD FOR PCR IDENTIFICATION OF FUNGAL INDOOR AIR CONTAMINANTS

Citation:

Dean, T R., D Betancourt, AND M Y. Menetrez*. A RAPID DNA EXTRACTION METHOD FOR PCR IDENTIFICATION OF FUNGAL INDOOR AIR CONTAMINANTS. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science BV, Amsterdam, Netherlands, 56(3):431-434, (2004).

Impact/Purpose:

Journal Article

Description:

Following air sampling, fungal DNA needs to be extracted and purified to a state suitable for laboratory use. Our laboratory has developed a simple method of extraction and purification of fungal DNA appropriate for enzymatic manipulation and polymerase chain reaction (PCR) applications. The methodology described is both rapid and cost effective for use with multiple fungal organisms

URLs/Downloads:

A Rapid DNA Extraction Method for PCR Identification of Fungal Indoor Air Contaminants   (PDF,NA pp, 1153 KB,  about PDF)

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Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Product Published Date: 03/01/2004
Record Last Revised: 03/24/2009
OMB Category: Other
Record ID: 80846

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL RISK MANAGEMENT RESEARCH LABORATORY

AIR POLLUTION PREVENTION AND CONTROL DIVISION

INDOOR ENVIRONMENT MANAGEMENT BRANCH