EPA Science Inventory

NITROTYROSINE ATTENUATES RSV-INDUCED INFLAMMATION IN AIRWAY EPITHELIAL CELLS

Citation:

Soukup, J M., Z. Li, S E. Becker, AND Huang, YuhChin T. NITROTYROSINE ATTENUATES RSV-INDUCED INFLAMMATION IN AIRWAY EPITHELIAL CELLS. Presented at American Thoracic Society Meeting, Seattle, Washington, May 16-21, 2003.

Description:

Nitrotyrosine attenuates RSV-induced inflammation in airway epithelial cells. Joleen Soukup, Zuowei Li, Susanne Becker and Yuh-Chin Huang. NHEERL, ORD, USEPA, RTP, North Carolina, CEMALB, University of North Carolina, Chapel Hill, North Carolina

Nitrotyrosine (NO2Tyr) is a naturally-occurring tyrosine analog whose concentration in bronchial lining fluid can increase during acute inflammation. Besides being a marker of oxidative-nitrosative stress, the biological function of nitrotyrosine remains unclear. Nitrotyrosine can be incorporated irreversibly into alpha-tubulin post-translationally by tubulin-tyrosine ligase, and impair microtubular function. Since microtubules may be involved in the intracellular processing of viral particles, we hypothesized that nitrotyrosine may attenutate inflammation induced by respiratory syncytial virus (RSV). Bronchial epithelial cells (BEAS-2B) were pre-incubated with or without NO2Tyr (10-100 microM) for 24 hrs prior to incubation with RSV at 0.1, 0.3 and 1.0 MOI for 72 hours. Release of IL-6, IL-8, RANTES and TNF-alpha were measured at 24, 48 and 72 hours by ELISA. Nitrotyrosine attenuated RSV-induced release of IL-8 and RANTES by 50%. The titers of RSV were lower at all time points in media containing NO2Tyr. NO2Tyr had no direct virucidal effect as measured by HEp-2 plaque assay nor did it affect cell viability. Immunoprecipitation and immunoblotting with alpha-tubulin antibody and anti-NO2Tyr antibody showed a time-dependent increase in nitration of alpha-tubulin in BEAS cultured in NO2Tyr-supplemented media. Fluorescent imaging showed decreased intracellular FITC-tagged RSV. These results indicate that NO2Tyr attenuated RSV-induced chemokine release and this inhibition was associated with nitrotyrosination of alpha-tubulin.
(This is an abstract of a proposed presentation and does not reflect EPA policy)



Record Details:

Record Type: DOCUMENT (PRESENTATION/ABSTRACT)
Start Date: 05/16/2003
Completion Date: 05/16/2003
Record Last Revised: 06/06/2005
Record Created: 03/18/2004
Record Released: 03/18/2004
Record ID: 80128

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LAB

HUMAN STUDIES DIVISION

CLINICAL RESEARCH BRANCH