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LIQUID CHROMATOGRAPHIC SEPARATION OF THE ENANTIOMERS OF TRANS-CHLORDANE, CIS-CHLORDANE, HEPTACHLOR, HEPTACHLOR EPOXIDE AND ALPHA-HEXACHLOROCYCLOHEXANE WITH APPLICATION TO SMALL-SCALE PREPARATIVE SEPARATION
Citation:
Champion Jr., W. L., J. Lee, A W. Garrison, J. C. DiMarco, A. Matabe, AND K. B. Prickett. LIQUID CHROMATOGRAPHIC SEPARATION OF THE ENANTIOMERS OF TRANS-CHLORDANE, CIS-CHLORDANE, HEPTACHLOR, HEPTACHLOR EPOXIDE AND ALPHA-HEXACHLOROCYCLOHEXANE WITH APPLICATION TO SMALL-SCALE PREPARATIVE SEPARATION. JOURNAL OF CHROMATOGRAPHY A 1024(1/2):55-62, (2004).
Impact/Purpose:
Extend existing model technologies to accommodate the full range of transport, fate and food chain contamination pathways, and their biogeographical variants, present in agricultural landscapes and watersheds. Assemble the range of datasets needed to execute risk assessments with appropriate geographic specificity in support of pesticide safety evaluations. Develop software integration technologies, user interfaces, and reporting capabilities for direct application to the EPA risk assessment paradigm in a statistical and probabilistic decision framework.
Description:
Analytical high-performance liquid chromatographic separations of the individual enantiomers of five polychlorinated compounds were obtained on polysaccharide stereoselective HPLC columns. The enantiomers of the pesticides trans-chlordane, cis-chlordane and heptachlor were separated on CHIRALCEL OD using a hexane mobile phase. The enantiomers of the heptachlor metabolite, heptachlor epoxide, were separated on CHIRALPAK AD using a methanol mobile phase. The enantiomers of -hexachlorocyclohexane (-HCH), were separated on CHIRALCEL OJ using a hexane/2-propanol mobile phase. Similar chromatographic conditions using preparative columns were used to isolate approximately 250 mg of each of the individual enantiomers. The purified individual enantiomers have been submitted for testing of their endocrine disruptor (ED) activity.