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BIODEGRADATION OF ORGANOPHOSPHORUS PESTICIDES BY SURFACE-EXPRESSED ORGANOPHOSPHORUS HYDROLASE. (R823663)
Citation:
Richins, R., I. Kaneva, A. Mulchandani, AND W. Chen. BIODEGRADATION OF ORGANOPHOSPHORUS PESTICIDES BY SURFACE-EXPRESSED ORGANOPHOSPHORUS HYDROLASE. (R823663). Analytical Chemistry. Nature Publishing Group, London, Uk, 15:984-987, (1997).
Description:
Organophosphorus hydrolase (OPH) was displayed and anchored onto the surface of
Escherichia coli using an Lpp-OmpA fusion system. Production of the fusion proteins in membrane
fractions was verified by immunoblotting with OmpA antisera. inclusion of the organophosphorus
hydrolase signal sequence was necessary for achieving enzymatic activity on the surface. More than
80% of the OPH activity was located on the cell surface as determined by protease accessibility
experiments. Whole cells expressing OPH on the cell surface degraded parathion and paraoxon very
effectively without any diffusional limitation, resulting in sevenfold higher rates of parathion
degradation compared with whole cells with similar levels of intracellular OPH. Immobilization of
these live biocatalysts onto solid supports could provide an attractive means for pesticide
detoxification in place of immobilized enzymes, affording a reduced diffusional barrier. (28
References)