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INTERACTION ANALYSES OF BINARY MIXTURES OF CARCINOGENIC PAHS USING MORPHOLOGICAL CELL TRANSFORMATION OF C3H1OT1/2CL8 MOUSE EMBRYO FIBROBLASTS IN CULTURE.
Citation:
Nesnow, S, C. R. Davis, M Pimentel, M J. Mass, G B. Nelson, AND J A. Ross. INTERACTION ANALYSES OF BINARY MIXTURES OF CARCINOGENIC PAHS USING MORPHOLOGICAL CELL TRANSFORMATION OF C3H1OT1/2CL8 MOUSE EMBRYO FIBROBLASTS IN CULTURE. POLYCYCLIC AROMATIC COMPOUNDS 21:31-42.
Description:
Studies of defined mixtures of carcinogenic polycyclic aromatic hydrocarbons (PAH) have shown three major categories of interactions: antagonism, synergism, and additivity depending on the biological model, tissue, route of exposure, and specific PAH. To understand the bases of these interactions we studied binary mixtures of benzo[a]pyrene (B[a]P) and dibenz[a,h]anthracene (DBA) in transformable C3H1OT1/2CI8 (C3H1OT1/2) mouse embryo fibroblast cells in culture. C3H1OT1/2 cells treated with binary mixtures of B[a]P and DBA gave less than additive morphological cell transformation than expected based on response additivity. These results were consistent with those reported in mice and rats on the antagonistic effects of B[a]P and DBA on tumorigenesis. 32P-Postlabeling DNA adduct studies revealed that DBA reduced B[a]P-DNA adduct levels by 47% with no effect on DBA-DNA adduct levels. This suggests that one mechanism for the inhibition of morphological cell transformation of binary mixtures of B[a]P and DBA is due to alterations in the metabolic activation of B[a]P.
Abstract Studies of defined mixtures of carcinogenic polycyclic aromatic hydrocarbons (PAH) have shown three major categories of interactions: antagonism, synergism, and additivity depending on the biological model, tissue, route of exposure, and specific PAH. To understand the bases of these interactions we studied binary mixtures of benzo[a]pyrene (B[a]P) and dibenz[a,h]anthracene (DBA) in transformable C3H1OT1/2CI8 (C3H1OT1/2) mouse embryo fibroblast cells in culture. C3H1OT1/2 cells treated with binary mixtures of B[a]P and DBA gave less than additive morphological cell transformation than expected based on response additivity. These results were consistent with those reported in mice and rats on the antagonistic effects of B[a]P and DBA on tumorigenesis. 32P-Postlabeling DNA adduct studies revealed that DBA reduced B[a]P-DNA adduct levels by 47% with no effect on DBA-DNA adduct levels. This suggests that one mechanism for the inhibition of morphological cell transformation of binary mixtures of B[a]P and DBA is due to alterations in the metabolic activation of B[a]P.