EPA Science Inventory

RESEARCH NOTE: AUTOFLUORESCENCE OF TOXOPLASMA GONDII OOCYSTS

Citation:

Lindquist, H. A., J. W. Bennett, J. D. Hester, M. W. Ware, J. P. Dubey, AND W. V. Everson. RESEARCH NOTE: AUTOFLUORESCENCE OF TOXOPLASMA GONDII OOCYSTS. JOURNAL OF PARASITOLOGY 89(4):865-867, (2003).

Description:

This is the first report of a blue autofluorescence as a useful characteristic in the microscopic identification of Toxoplasma gondii oocysts. This autofluorescence appears to be of high intensity. Similar to the autofluorescence of related coccidia, the oocysts glow pale blue when illuminated with an ultraviolet light source and viewed with the correct (UV) excitation and emission filter set.

Purpose/Objective:

1) Refine new, practical methods for the detection of CCL-related and emerging waterborne human protozoa.

2) Perform field tests of devices or methods that have been developed under this task.

3) Evaluate these methods or devices in a variety of water matrices and parasite concentrations.

This work in this task supports CCL2 and 3 and is expected to be completed by 9/07.

Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Start Date: 09/01/2003
Completion Date: 09/01/2003
Record Last Revised: 12/22/2005
Record Created: 10/20/2003
Record Released: 10/20/2003
Record ID: 66422

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL EXPOSURE RESEARCH LAB

MICROBIOLOGICAL AND CHEMICAL EXPOSURE ASSESSMENT DIVISION

BIOHAZARD ASSESSMENT RESEARCH BRANCH