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INDUCTION OF AN ESTROGEN-RESPONSIVE REPORTER GENE IN RAINBOW TROUT HEPATOMA CELLS (RTH 149) AT 11 OR 18 DEGREES C
Citation:
Hornung, M. W., G T. Ankley, AND P. K. Schmieder. INDUCTION OF AN ESTROGEN-RESPONSIVE REPORTER GENE IN RAINBOW TROUT HEPATOMA CELLS (RTH 149) AT 11 OR 18 DEGREES C. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. SETAC Press, Pensacola, FL, 22(4):866-871, (2003).
Description:
A reporter gene assay in a cultured rainbow trout cell line was used to determine the influence of temperature on the expression of an estrogen-responsive gene. Rainbow trout hepatoma cells (RTH 149) incubated at 11 or 18 degrees C were co-transfected with an estrogen-responsive luciferase reporter plasmid, and a plasmid containing a constitutively expressed rainbow trout estrogen receptor. - - - Although there may be slight differences in responses between the two temperatures tested here, this assay can be used to effectively determine the relative estrogenic activity of chemicals within the physiological temperature range of rainbow trout.