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THE MURINE LOCAL LYMPH NODE ASSAY: AN ALTERNATIVE TEST METHOD FOR THE EVALUATION OF THE POTENTIAL FOR CHEMICALS TO ELICIT ALLERGIC CONTACT DERMATITIS
Sailstad, D M. THE MURINE LOCAL LYMPH NODE ASSAY: AN ALTERNATIVE TEST METHOD FOR THE EVALUATION OF THE POTENTIAL FOR CHEMICALS TO ELICIT ALLERGIC CONTACT DERMATITIS. LAB ANIMAL. Nature Publishing Group, London, Uk, 31(7):36-41, (2002).
The process that a new toxicology test method must undergo to attain acceptance and regulatory implementation may seem daunting. As the first test method to undergo Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM) review, the local lymph node assay (LLNA) is the guide for future test method evaluation and eventually acceptance. This article is not written to provide an exhaustive review of the LLNA, the ICCVAM process and current regulatory or scientific status of the assay, but to briefly describe the application, mechanism, LLNA protocol and the review process. Additionally, a brief discussion is included of some of the lessons learned along with the benefits that both the LLNA and ICCVAM offer the regulatory, scientific and animal welfare communities.