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LOW PRESSURE ULTRAVIOLET STUDIES FOR INACTIVATION OF GIARDIA MURIS CYSTS
Citation:
Hayes*, S, E Rice*, M W. Ware, AND F W. Schaefer III. LOW PRESSURE ULTRAVIOLET STUDIES FOR INACTIVATION OF GIARDIA MURIS CYSTS. Godfree, A.F. (ed.), JOURNAL OF APPLIED MICROBIOLOGY. Society for Applied Microbiology, 94:54-59, (2003).
Description:
Cysts of Giardia muris were inactivated using a low pressure ultravolet (UV) light source. Cyst viability was detemined by both in vitro excystation and animal infectivity. Cyst doeses were counted using a flow cytometer for the animal infectivity experiments. Using in vitro excystation as the viability indicator, fluences as high as approximately 200 mJ/cm(2) did not prevent some cysts from excysting, thus verifying earlier work. Using animal infectivity, UV fluences of 1.4, 1.9 and 2.3 mJ/cm(2) yielded log(10) reductions ranging from 0.3 to greater than or equal to 4.4. Results indicate in vitro excystation is not a reliable indicator of G. muris cyst viability after UV disinfection.