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METHODS FOR THE SPIRAL SALMONELLA MUTAGENICITY ASSAY INCLUDING SPECIALIZED APPLICATIONS
Citation:
Claxton, L D., V. S. Houk, AND S. Warren. METHODS FOR THE SPIRAL SALMONELLA MUTAGENICITY ASSAY INCLUDING SPECIALIZED APPLICATIONS. MUTATION RESEARCH 488(3):241-257, (2001).
Description:
ABSTRACT
An automated approach to bacterial mutagenicity testing--the spiral Salmonella assay--was developed to simplify testing and to reduce the labor and materials required to generate dose-responsive mutagenicity information. This document provides the reader with an overview of the spiral assay and a discussion of its application for examining the mutagenic potential of pure compounds, complex environmental mixtures, and interactive effects. Guidelines for performing a routine spiral assay are presented, and alternative test methods intended to overcome a variety of technical difficulties (such as restricted sample availability, sample viscosity or volatility, etc.) are recommended. Methods for the computerized analysis of data and the interpretation of results are discussed.