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INTERACTIONS OF CHLORPYRIFOS AND METHYL MERCURY: A MECHANISTIC APPROACH TO ASSESS CHEMICAL MIXTURES
Citation:
Steevens, J. A. AND W H. Benson. INTERACTIONS OF CHLORPYRIFOS AND METHYL MERCURY: A MECHANISTIC APPROACH TO ASSESS CHEMICAL MIXTURES. MARINE ENVIRONMENTAL RESEARCH 50(1-5):113-117, (2001).
Description:
The mechanism of interaction between chlorpyrifos, an organophosphate insecticide, and methyl mercury was assessed utilizing the amphipod, Hyalella azteca. Previous studies have demonstrated that chlorpyrifos and methl mercury interact additively with survival as the endpoint. In addition, exposure to chlorpyrifos and methyl mercury increased the accumulation and decreased the elimination of methyl mercury. To further examine the mechanism responsible for these interactions, the in vivo and in vitro inhibition of acetylcholinesterase enzyme activity was assessed following exposure to methyl mercury and chlorpyrifos. In vivo, methyl mercury antagonized the effects of chlorpyrifos on acetylcholinesterase inhibition. Additional studies demonstrated that methyl mercury did not affect the in vitro bioactiviation of chlorpyrifos or the subsequent inhibition of acetylcholinesterase enzyme activity. Chemical-chemical interactions were examined utilizing chromatographic techniques and suggested the formation of chlorpyrifos-methyl mercury complex. The formation of this complex may result in increased accumulation of methyl mercury, apparent additive toxicity, and protection against chlorpyrifos-mediated acetylcholinesterase inhibition.