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ARRAY TECHNOLOGY AS A TOOL TO MONITOR EXPOSURE OF FISH TO XENOESTROGENS
Citation:
Larkin, P., G G. Akland, L C. Folmar, M J. Hemmer, P. Poston, H. Lee, AND N D. Denslow. ARRAY TECHNOLOGY AS A TOOL TO MONITOR EXPOSURE OF FISH TO XENOESTROGENS. Pollutant Responses in Marine Organisms, Plymouth Marine Laboratory, Plymouth, Devon, UK, July 10-13, 2001.
Description:
Use of DNA Macroarrays to Evaluate the Effects of Environmental Estrogens on Wildlife. In: Proceedings of the 2nd International Conference on Pharmaceuticals and Endocrine Disrupting Chemicals in Water, 9-11 October 2001, Minneapolis, MN. National Ground Water Association, Westerville, OH. 9 p. (ERL,GB 1143).
A variety of anthropogenic chemicals are capable of binding to the estrogen receptor of vertebrate species. Binding of these compounds can interfere with homeostasis by disrupting normal gene expression patterns. The purpose of this study was to investigate the feasibility of applying array technology as a monitoring tool for detecting the presence and distribution of estrogenic compounds in coastal habitats using sheephead minnows as our model. cDNA clones that were isolated from differential display, including vitellogenin a and b, vitelline envelope protein (ZP2), and transferrin, among others, were spotted on the macroarray. The results of these experiments demonstrate a characteristic expression pattern of estrogen responsive genes in sheepshead minnows exposed to 17 b-estradiol (E2).