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CONCENTRATION AND PROCESSING OF WATERBORNE VIRUSES BY POSITIVE CHARGE 1MDS CARTRIDGE FILTERS AND ORGANIC FLOCCULATION
Citation:
Fout, G S., D R. Dahling, AND R S. Safferman. CONCENTRATION AND PROCESSING OF WATERBORNE VIRUSES BY POSITIVE CHARGE 1MDS CARTRIDGE FILTERS AND ORGANIC FLOCCULATION. U.S. Environmental Protection Agency, Washington, DC, EPA/600/4-84/013 (N14), 2001.
Impact/Purpose:
Develop sensitive techniques to detect and identify emerging human waterborne pathogenic viruses and viruses on the CCL.
Determine effectiveness of viral indicators to measure microbial quality in water matrices.
Support activities: (a) culture and distribution of mammalian cells for Agency and scientific community research needs, (b) provide operator expertise for research requiring confocal and electron microscopy, (c) glassware cleaning, sterilization and biological waste disposal for the Cincinnati EPA facility, (d) operation of infectious pathogenic suite, (e) maintenance of walk-in constant temperature rooms and (f) provide Giardia cysts.
Description:
This chapter describes the most widely used virus adsorption-elution (VIRADEL) method for recovering human enteric viruses from water matrices (Fout et al., 1996). The method takes advantage of postively charged cartridge filters to concentrate viruses from water. The major advantage of methods that use these filters over those described in Chapters 5 and 6 that use negatively charged filters is that additives are not normally needed to achieve virus adsorption to filters.