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MICROSATELLITE DNA VARIATION IN TWO FATHEAD MINNOW (PIMEPHALES PROMELAS) STOCKS
Citation:
BrownAugustine, M. C., S. I. Guttman, AND M J. Bagley. MICROSATELLITE DNA VARIATION IN TWO FATHEAD MINNOW (PIMEPHALES PROMELAS) STOCKS. Presented at Society of Environmental Toxicology and Chemistry, Austin, TX, November 8-13, 2003.
Impact/Purpose:
The objective of this task is to develop molecular indicators to evaluate the integrity and sustainability of aquatic fish, invertebrate, and plant communities (GPRA goal 4.5.2). Specifically, this subtask aims to evaluate methods for the measurement of:
fish and invertebrate community composition, especially for morphologically indistinct (cryptic) species
population genetic structure of aquatic indicator species and its relationship to landscape determinants of population structure (to aid in defining natural assessment units and to allow correlation of population substructure with regional stressor coverages)
genetic diversity within populations of aquatic indicator species, as an indicator of vulnerability to further exposure and as an indicator of cumulative exposure
patterns of temporal change in genetic diversity of aquatic indicator species, as a monitoring tool for establishing long-term population trends.
Description:
Adverse effects on more than 2000 species of fish in the U.S. and Canada are estimated by sensitvity results of fathead minnow (Pimephales promelas) acute toxicity tests. Whether survival and susceptibility to toxicants are influenced by genetic variation is still under question. This work is part of a larger study to assess the importance of genetic variation in the fathead minnow and to quantify genetic components of copper resistance. Two populations of fathead minnows were examined for microsatellite DNA variation. Genetic variation differed between and within stocks, as shown by number of alleles, expected heterozygosity, and gene frequency.