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THE APPLICATION OF MASS SPECTROMETRY TO PROTEIN ANALYSIS
Citation:
Shoemaker, J A. THE APPLICATION OF MASS SPECTROMETRY TO PROTEIN ANALYSIS. Presented at NHEERL Presenation on Proteomics, Research Triangle Park, NC, July 22, 2003.
Impact/Purpose:
This particular task is comprised of 4 subtasks: 1.) Characterization of Potential Viral Biomarkers by Mass Spectrometry; 2.) Characterization of Parasites by Mass Spectrometric Techniques;
3.) Rapid Discrimination of Bacterial Indicators of Fecal Contamination and Bacterial Pathogens by Mass Spectrometric Techniques; and 4.) Investigation of Aeromonas Virulence Factors Using Mass Spectrometry.
The purpose of this research project is to use mass spectrometric techniques, such as electrospray ionization (ESI), capillary electrophoresis (CE) and matrix assisted laser desorption ionization (MALDI) mass spectrometry, to provide "protein mass fingerprinting" and protein sequencing information for viruses, bacteria and protozoa that cause waterborne disease. These protein mass fingerprinting libraries will be evaluated to determine whether mass spectrometric techniques can identify protein fingerprints related to the infectivity/viability of selected microorganisms and whether they can differentiate between infective / non-infective genus and strains of the selected microorganisms. The characteristic proteins identified by mass spectrometry as markers of infectivity/viability or strain differentiation can then be used to develop more sensitive microbiological drinking water methods.
Description:
The purpose of this presentation is to give our NHEERL collaborators a brief introduction to the use of mass spectrometric (MS) techniques in the analysis of proteins. The basic principles of electrospray ionization and matrix-assisted laser desorption ionization will be discussed, as well as the use of tandem mass spectormetry for de novo sequencing of novel proteins. This information will aid our collaborators in understanding the proteomic data we will be providing them on allergenic fungal proteins extracted from Metarhizium Anisopliae.