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SPECIES DIFFERENCES IN ANDROGEN AND ESTROGEN RECEPTOR STRUCTURE AND FUNCTION AMONG VERTEBRATES AND INVERTEBRATES FOR INTERSPECIES EXTRAPOLATION OF ENDOCRINE DISRUPTING CHEMICALS
Citation:
Wilson, V S., G T. Ankley, M. P. Gooding, P. D. Reynolds, N C. Noriega, M. Cardon, L. J. Guillette, P. Hartig, G. A. LeBlanc, J. Welch, C. S. Lambright, J. Thorton, AND L. E. Gray Jr. SPECIES DIFFERENCES IN ANDROGEN AND ESTROGEN RECEPTOR STRUCTURE AND FUNCTION AMONG VERTEBRATES AND INVERTEBRATES FOR INTERSPECIES EXTRAPOLATION OF ENDOCRINE DISRUPTING CHEMICALS. Presented at 2002 SETAC Annual Meeting, Salt Lake City, UT, November 16-20, 2002.
Description:
In vitro screening assays designed to identify hormone minics or antagonists, including the EDSTAC Tier 1 Screening (TIS) Battery, typically use only mammalian estrogen (ER) and androgen receptors (AR). However, there is uncertainty concerning species differences in binding affinities of toxicants for steroid receptors because gene and protein structures are not identical among animals. - - - Although significant differences in affinities or chemicals have yet to be identified between rtAR and hAR, some of our results with xenobiotic AR ligands differ from reports in the literature which assessed AR binding is tissue cytosolic preparations. These studies will facilitate the identification of chemicals that affect reproduction and development in multiple species via stetoid hormone receptors and ultimately impact on the design of screening batteries for endocrine disrupting chemicals.