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UTILIZATION OF TREATABILITY AND PILOT TESTS TO PREDICT CAH BIOREMEDIATION
Citation:
Lutes, C. C., D. Liles, M. A. Hansen, J. Burdick, S. Suthersan, J. E. Hansen, D H. Kampbell*, AND D. McInnes. UTILIZATION OF TREATABILITY AND PILOT TESTS TO PREDICT CAH BIOREMEDIATION. Presented at ESTCE/SERDP: Partners in Env. Tech. Mtg, Washington, DC, 11/27-29/2001.
Description:
Multiple tools have been suggested to help in the design of enhanced anaerobic bioremediation systems for CAHs:
- Extensive high quality microcosm testing followed by small-scale, thoroughly observed field pilot tests (i.e., RABITT Protocol, Morse 1998)
- More limited microcosm testing (Findlay and Fogel 2000)
- Site screening using quantitative and qualitative microbiology techniques that do not directly measure contaminant disappearance (i.e. plate counts, direct counts, Phospholipid Fatty Acid [PLFA] analysis, Denaturing Gradient Gel Electrophoresis [DGEE])
- Site screening based on geochemical and hydrological information followed by limited field pilot testing (Lenzo in Nyer 2001)
-Pump tests
This presentation will be based primarily on data generated at an ESTCP/AFCEE co-funded field demonstration test program at Vandenberg AFB, Lompoc, CA, in which five of the following approaches have been employed:
- Site screening based on geochemical and hydrological information (complete)
- Microcosm testing (one round complete, additional round ongoing)
- Quantitative and qualitative microbiological screening using PLFA analysis and DGEE (complete)
- Extensive pilot testing (began February 2001, preliminary positive biogeochemical results received, extensive CAH data will be in hand by the time of the presentation)
- Pump tests (complete)
In addition, multiple study designs for microcosm testing were evaluated using a sample from the same location. Thus, this site forms an ideal basis for evaluating the information content of these different tools and for recommending which tools should be used in routine system design.