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HPLC SEPARATION OF CHIRAL ORGANOPHOSPHORUS PESTICIDES ON POLYSACCHARIDE CHIRAL STATIONARY PHASES
Citation:
Ellington, J J., J J. Evans, K. B. Prickett, AND W. L. Champion. HPLC SEPARATION OF CHIRAL ORGANOPHOSPHORUS PESTICIDES ON POLYSACCHARIDE CHIRAL STATIONARY PHASES. Presented at 22nd Annual Society of Environmental Toxicology and Chemistry Meeting, Baltimore, MD, November 11-15, 2001.
Impact/Purpose:
Elucidate and model the underlying processes (physical, chemical, enzymatic, biological, and geochemical) that describe the species-specific transformation and transport of organic contaminants and nutrients in environmental and biological systems. Develop and integrate chemical behavior parameterization models (e.g., SPARC), chemical-process models, and ecosystem-characterization models into reactive-transport models.
Description:
High-performance liquid chromatographic separation of the individual enantiomers of 12 organophosphorus pesticides (OPs) were obtained on polysaccharide chiral HPLC columns using an alkane-alcohol mobile phase. The OP pesticides were crotoxyphos, dialifor, dyfonate, fenamiphos, fensulfothion, isofenphos, malathion, methamidophos, profenofos, ruelene, tokuthion and trichloronate. The enantiomers of the twelve OPs were baseline resolved on at least one of the following columns: CHIRALPAK ADTM, CHIRALPAK ASTM, CHIRALCEL ODTM, CHIRALCEL OJTM, and CHIRALCEL OGTM. In continued method development,theseparation of the enantiomers of the twelve OPs was investigated more extensively on CHIRALCEL OJ to determine whether the mobile phase composition, flow rate and column temperature could be optimized to yield at least partial separation of the enantiomers. Chromatographic conditions were found that gave either baseline or near baseline separations of the enantiomers of the twelve OPs on the CHIRALCEL OJ column. Enzymatic degradation of several of the OPs was determined using a partially purified enzyme from Escherichia coli. The enzymatic degradation of the OP enantiomers was followed by HPLC using a CHIRALCEL OJTM column. The enzyme quickly degraded crotoxyphos enantiomers while dyfonate degradation took considerably longer. The enantiomers of both OPs were degraded at different rates. The enzyme did not degrade dialifor.