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IN VIVO KINETICS OF PHENYLGLUCURONIDE, A PHASE II CONJUGATE OF PHENOLE, IN BLOOD AND URINE OF RAINBOW TROUT
Citation:
Nichols, J W., A D. Hoffman, C. T. Jenson, D. Kuehl, G J. Lien, AND J M. McKim. IN VIVO KINETICS OF PHENYLGLUCURONIDE, A PHASE II CONJUGATE OF PHENOLE, IN BLOOD AND URINE OF RAINBOW TROUT. Presented at Society of Toxicology National Meeting, San Francisco, CA, March 25-29, 2001.
Description:
The kinetics of phenylglucuronide (PG) in blood and urine of spinally-transected rainbow trout were investigated using microdialysis sampling techniques. Trout weighing 0.9 to 1.3 kg were dosed continuously with PG for an additional 48 h. PG could not be detected in expired branchial water suggesting that it was not eliminated across the gills. An absence of phenol in blood indicated that there was no conversion to the parent compound. Mass-balance calculations showed that 93% of infused PG was eliminated in urine during the depuration period. The kinetics of PG in blood and urine were similar. Based on peak values, the mean concentration of PG in urine was 3.1 times greater than that in blood, suggesting that PG was actively secreted in the kidney. The kinetics of PG in blood were adequately described by a one-compartment renal clearance model. The mean fitted clearance constant was 13.5 ml/kg/h, which is twice the estimated GFR and about 2% of total renal blood flow. The apparent volume of distribution ranged from 200 to 300 ml, which is close to the extracellular water volume. This is the first known kinetic study of an infused Phase II conjugated metabolite in fish.