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TYROSINASE-BASED CARBON PASTE ELECTRODE BIOSENSOR FOR DETECTION OF PHENOLS: BINDER AND PRE-OXIDATION EFFECTS
Citation:
Rogers, K R., J. Y. Becker, AND J Cembrano. TYROSINASE-BASED CARBON PASTE ELECTRODE BIOSENSOR FOR DETECTION OF PHENOLS: BINDER AND PRE-OXIDATION EFFECTS. Presented at 8th International Meeting on Chemical Sensors, Basel, Switzerland, July 2-5, 2000.
Impact/Purpose:
The overall objective of this task is to develop scientifically sound sampling and bioanalytical approaches for screening and monitoring of hazardous wastes. These techniques are expected to provide the Agency with improved screening and field portable methods to characterize, reduce, and control risk to human health and the environment. Specific objectives will include development and characterization of the following concepts:
SPMDs for passive accumulation of TICs
Bioassays for toxic and genotoxic compounds
MIPs for volatile and semivolatile toxic organics
Rapid screening assays using the previously listed components.
Description:
Tyrosinase-based carbon paste electrodes are evaluated with respect to the viscosity and polarity of the binder liquids. The electrodes constructed using a lower viscosity mineral oil or paraffin wax oil yielded a greater response to phenol and catechol than those using the higher viscosity oils of similar chemical composition. Despite its relatively high viscosity, the use of silicone oil Si PS 086 as carbon paste binder resulted in a 2 fold increase over the silicone oil Si AR 200 and a 4-6 fold increase (over electrodes using mineral oils) in responses to phenol, p-chlorophenol, and p-cresol. Carbon paste electrodes using a series of (DC200) silicone oils ranging in viscosity from 10 to 60,000 mPa.s were constructed and evaluated. A significantly enhanced response was observed using a binder of intermediate viscosity (100 mPa.s). Also reported is the enhanced enzyme electrode response to phenol and catechol resulting from pre-oxidation of these substrates using a plain carbon paste electrode in a series (in-line) dual electrode configuration.
The U.S. Environmental Protection Agency (EPA), through its Office of Research and Development (ORD), funded this research and approved this abstract as a basis for an oral presentation. The actual presentation has not been peer reviewed by EPA.