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ACCELERATED SOLVENT EXTRACTION OF ARSENICALS FROM SEAFOOD MATRICES WITH ION CHROMATOGRAPHY AND ICP-MS DETECTION
Citation:
Creed, J T., P A. Gallagher, X. Wei, C A. Schwegel, R. M. Lorenzana, AND I. Chamberlain. ACCELERATED SOLVENT EXTRACTION OF ARSENICALS FROM SEAFOOD MATRICES WITH ION CHROMATOGRAPHY AND ICP-MS DETECTION. Presented at 4th International Conference on Arsenic Exposure and Health Effects, San Diego, CA, June 18-22, 2000.
Impact/Purpose:
To develop an arsenic speciation protocol for the analysis of dietary seafoods to be used to support fish advisories, improve relative source (water versus diet) contribution for arsenic and provide improved dietary exposure estimates in future epidemiology (EPI) studies.
Description:
The two major sources of arsenic exposure are water and diet. Dietary exposure is considerably more difficult to assess because of the diversity of arsenicals present in dietary matrices coupled with species dependent toxicity of arsenic. Dietary arsenic assessments are further complicated by an incomplete understanding of all analytical factors which assure species specific integrity from extraction to detection and variation in extraction efficiencies. Near quantitative extractions may be achieved on a Standard Reference Material while the extraction of similar matrices produces extraction efficiencies of <25%. In this case, 75% of arsenicals remains unextracted. This type of variance in extraction efficiency within the same type of matrix is one reason extraction of arsenicals from dietary matrices is still an active area of research. One approach to extraction of arsenicals from seafood sources is accelerated solvent extraction (ASE). This approach allows for optimization of solvent, pressure, temperature and static time. The ASE will be evaluated in terms of its ability to quantitatively extract arsenicals from seafoods using conventional solvents while maintaining species integrity. The ASE is a semi-automated extraction device in which the sample is loaded into a stainless steel cell after it has been dispersed in a dispersion media. The appropriate choice of cell components and dispersion media will be presented with an emhasis on QC samples. This evaluation wll include chromatographic concerns resulting from addition of arsenosugars to potential list of extractable arsenicals and use of ICP-MS as a detector.