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EFFECTS OF ENDOGENOUS AND XENOBIOTIC CHEMICALS ON INSULIN-LIKE GROWTH FACTOR (IGF) INDUCTION IN THE SHEEPSHEAD MINNOW
Citation:
Gillis, L. D., I. Knoebl, AND L C. Folmar. EFFECTS OF ENDOGENOUS AND XENOBIOTIC CHEMICALS ON INSULIN-LIKE GROWTH FACTOR (IGF) INDUCTION IN THE SHEEPSHEAD MINNOW. Presented at SETAC 20th Annual Meeting, Philadelphia, PA, November 14-18, 1999.
Description:
EPA has been mandated by Congress to develop methods to assess the health and ecological effects of "endocrine-disrupting chemicals" in the environment. To date, EPA's focus has been on reproductive impairment and thyroid function. Here, we describe an in vivo method for growth assessment using an antisense probe to measure IGF-1 expression associated with injections of exogenous growth hormone (GH), triiodothyronine (T3), insulin, 17b-estradiol (E2) or an aquatic exposure to estradiol or chlorpyrifos. This study in a teleost model should aid in the development of screening methods for evaluating the physiological impact of environmentally significant chemicals on somatic growth. The analysis of growth at the molecular level may provide environmental screening protocols which address xenobiotic chemical effects more efficiently than current methods.