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CHLORINE DISINFECTION STUDIES OF ENCEPHALITOZOON (SEPTATA) INTESTINALIS
Citation:
Rice*, E W., C H. Johnson*, D. W. Naumovitz, M. M. Marshall, C. B. Plummer, AND C. R. Sterling. CHLORINE DISINFECTION STUDIES OF ENCEPHALITOZOON (SEPTATA) INTESTINALIS. Annual Meeting of American Society for Microbiology, Poster Presentation, Chicago, IL, 05-99.
Description:
A reproducible standardized assay was designed to determine two infective doses for E.intestinalis, the TCID50 and the MID. These doses can be used to assess the potential effectiveness of chlorine disinfection and can also be used to assess other disinfection parameters and antibiotic susceptibility of microsporidia. Our results show that chlorine at 2mg/l inactivated E.intestinalis spores by >3 logs in chlorine-demand-free laboratory water at 16 minutes. Studies using laboratory waters are helpful to determine disinfection parameters and ranges, but no definitive conclusions can be draw about chlorine's effect on microsporidia in the environment until source water studies have been performed. Spectrophotometeric methods (%T) may be useful for enumeration of E.intestinalis spores.