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XENOBIOTIC METHODS DEVELOPMENT FOR HUMAN EXPOSURE ASSESSMENT RESEARCH

Contact

http://cfpub.epa.gov/si/si_public_comments.cfm

Impact/Purpose:

The objective of this task is to develop state-of-the-art methods for measuring xenobiotic compounds, to include the isolation of the analyte from the appropriate matrix (extraction), preconcentration (typically sorbent-based), and analysis via GC/MS and/or LC/MS. Once established, these methods will be applied in small scale pilot studies or demonstration projects. Particular emphasis will be placed on methods which are readily transferable to other laboratories, including those within the Human Exposure and Atmospheric Sciences Division (HEASD), the National Exposure Research Laboratory (NERL), other EPA Laboratories, Program Offices, Regions, and academic institutions.

Specific objectives of this task include the following:

1) Development of GC/MS and LC/MS methods for the measurement of key xenobiotic compounds and their metabolites (to include the pyrethroid pesticides, perfluorinated organic compounds, and the BFRs) in relevant environmental and biological matrices.

2) Development of efficient low cost methods for the extraction and clean up of these compounds collected from relevant matrices.

3) Determination of xenobiotic compound and metabolite concentrations in samples derived from laboratory and field monitoring studies to help assess exposures and evaluate associated risks.

Description:

Biomarkers from blood, breath, urine, and other physiological matrices can provide useful information regarding exposures to environmental pollutants. Once developed and applied appropriately, specific and sensitive methods can often provide definitive data identifying the various sources of exposure; the magnitude of exposure; and the uptake, elimination, and body burden of specific compounds. These data can be essential for the appropriate evaluation of the risks incurred with particular exposures.

This task focuses on the development and application of gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS) based biomarker methods for use in exposure assessment research. Emphasis will be placed on the development of methods to investigate exposures to current- and future-use pesticides, key perfluorinated organic compounds (e.g., perfluorooctane sulfonate [PFOS] and perfluorooctanoic acid [PFOA]), brominated fire retardants (BFR) and other high priority compounds identified by State, Regional, and Program Offices.

Record Details:

Record Type:PROJECT
Start Date:10/01/2001
Projected Completion Date:09/01/2006
Record ID: 56151