Citation:

Teska, J., E. Shotts, AND T. Hsu. AUTOMATED BIOCHEMICAL IDENTIFICATION OF BACTERIAL FISH PATHOGENS USING THE ABBOTT QUANTUM II. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-89/026 (NTIS PB90100827), 1989.

Description:

The Quantum II, originally designed by Abbott Diagnostics for automated rapid identification of members of Enterobacteriaceae, was adapted for the identification of bacterial fish pathogens. he instrument operates as a spectrophotometer at a wavelength of 492.600 nm. ample cartridge containing 20 inoculated biochemical chambers is inserted in the path of the analyzing beam. eactions are converted into a 7-digit octal biocode, relayed via a sensor to the memory module, and compared to biocodes preprogrammed in the memory. n identification is then printed. resently, the Quantum II is capable of identifying human strains of Aeromonas hydrophila and Edwardsiella tarda. his study was initiated to determine the feasibility of expanding the use to the Quantum II to include identification of bacterial fish pathogens. Ten to 50 isolates of Edwardsiella ictaluri, Serratia liquefaciens. Yersinia rukert, Aeromonas hydrophila, typical Aeromonas salmoncida, and atypical Aeromonas salmoncida were utilized to determine optimal incubation conditions, relative stability of the biochemicals, and ability to obtain consistent biocide numbers. fter sorting the octal biocides from the 169 isolates into groupings using a cluster analysis technique, it was shown by a Chisquare goodness of fit test that isolates of a given species were sorted into the same cluster group at a frequency of at least 99%. esults of this study illustrate the usefulness of the Quantum II BID system for the identification of bacterial fish pathogens not contained in the system's memory module.

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