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APPLICATION OF THE MICRO-FORWARD MUTATION ASSAY TO ASSESS MUTAGENICITY OF AIRBORNE PARTICULATES IN INDOOR
Citation:
Takagi, Y., S. Goto, M. Murata, H. Matsushita, AND J. Lewtas. APPLICATION OF THE MICRO-FORWARD MUTATION ASSAY TO ASSESS MUTAGENICITY OF AIRBORNE PARTICULATES IN INDOOR. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-88/542 (NTIS PB91109231).
Description:
Validity test of the micro-forward mutation assay using Salmonella typhimurium strain TM677 was carried out using benzene-ethanol extracts from airborne particulates as test materials. ensitivity of this assay in the presence and absence of 5-9 mix was five to ten times higher than the pre-incubation method, a, modified method of Ames's original method, using Salmonella typhimurium strains TA98 and TA1OO. his means that the virtual sensitivity of the micro-forward mutation assay is ten times or more higher than the pre-incubation method, because two or more strains are necessary in the latter method. epeatability of the micro-forward mutation assay was nearly same with that of the pre-incubation method. hat is, the coefficient of variation of mutagenic frequency of the particulate extracts was about 10%. This forward mutation assay was applied to airborne particulates indoors, and proved to be able to measure easily the mutagenic activity of the extracts from, particulates collected by only 30 m3 air sampling. ome indoor pollutants, especially ones collected in a room, in which cigarette smoking was done, showed higher mutagenic activity than that of outdoor sale. article size dependency of the mutagenic activity could also measure by this method using air particulates collected by only 90 m3 -air sampling with an Andersen/Low Pressure Impactor. he highest mutagenic activity was found in the particulates of 0.52-0.33 um in size. hese results suggest strongly that the micro-forward mutation assay is a useful tool for survey mutagenicity of indoor environment.