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DIFFERENCES IN ARACHIDONIC ACID METABOLISM BY HUMAN MYELOMONCYTIC CELL LINES
Madden, M., S. Becker, H. Koren, AND M. Friedman. DIFFERENCES IN ARACHIDONIC ACID METABOLISM BY HUMAN MYELOMONCYTIC CELL LINES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-92/280 (NTIS PB92217215).
The production of arachidonic acid metabolites by the HL60, ML3, and U937 human phagocyte cell lines were determined after incubation with interferongamma (IFNg; 500 U/ml) or vehicle for 4 days. ells were prelabeled with tritiated arachidonic acid for 4 hours, and media supernatants analyzed by high performance liquid chromatography. one of the cell lines produced H-AA metabolites in large amounts during an unstimulated, basal release period (30 or 60 min). n response to 10 uM calcium ionophore A23187 incubation (30 min), undifferentiated and IFNg-differentiated HL60 cells formed both cyclooxygenase products thromboxane and prostaglandins) and lipoxygenase products (leukotrienes and hydroxyeicosatetraenoic acids). In contrast to the HL60 cells, IFNg-differentiated U937 cells formed primarily cyclooxygenase products, while undifferentiated and IFNg-differentiated ML3 cells did not form any 3H-AA metabolites in response to A23187. hese data point out the need to carefully select a cell line for use in a phagocyte assay system when cyclooxygenase and/or lipoxygenase products could influence the assay results.
Record Details:Record Type: DOCUMENT (REPORT)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT