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EFFICACY OF B-GLUCURONIDASE ASSAY FOR IDENTIFICATION OF ESCHERICHIA COLI BY THE DEFINED SUBSTRATE TECHNOLOGY
Citation:
Rice, E.W., M. Allen, AND S. Edberg. EFFICACY OF B-GLUCURONIDASE ASSAY FOR IDENTIFICATION OF ESCHERICHIA COLI BY THE DEFINED SUBSTRATE TECHNOLOGY. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/407 (NTIS PB91109876), 1990.
Description:
In 1976, Kilian and Bulow described the association of B-glucuronidase with the genus Escherichia (97% positive) and suggested that a B-glucuronidase assay would be a useful identification test. ince that report, papers about the sensitivity and specificity of this enzyme for the identification of Escherichia coli from clinical sources, food, seawater, potable-water supplies, and various environmental sources have appeared. tudy was undertaken to determine the efficacy and specificity of the defined-substrate technology B-glucuronidase (Colilert) assay for the identification of this species from fecal samples. otal of 460 humans, 105 cow, and 55 horse E. coli isolates were tested. esults showed 95.5% B-glucuronidase-positive isolates in 24 h and 99.5% positive after 28 h of incubation. nly one E. coli isolate was negative. here were no significant differences in the percentage of B-glucuronidase-positive isolates among the human or animal isolates. here were no non-E. coli isolates that were positive. ll subjects carried B-glucuronidase-positive E. coli.
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EFFICACY OF B-GLUCURONIDASE ASSAY FOR IDENTIFICATION OF ESCHERICHIA COLI BY THE DEFINED SUBSTRATE TECHNOLOGY