Science Inventory

NUCLEOTIDE SEQUENCING AND TRANSCRIPTIONAL MAPPING OF THE GENES ENCODING BIPHENYL DIOXYGENASE, A MULTICOMPONENT POLYCHLORINATED-BIPHENYL-DEGRADING ENZYME IN PSEUDOMONAS STRAIN LB400

Citation:

Erickson, B. AND F. Mondello. NUCLEOTIDE SEQUENCING AND TRANSCRIPTIONAL MAPPING OF THE GENES ENCODING BIPHENYL DIOXYGENASE, A MULTICOMPONENT POLYCHLORINATED-BIPHENYL-DEGRADING ENZYME IN PSEUDOMONAS STRAIN LB400. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/299 (NTIS PB93222909), 1992.

Description:

The DNA region encoding biphenyl dioxygenase, the first enzyme in the biphenyl-polychlorinated biphenyl degradation pathway of Pseudomonas species strain LB400, was sequenced. ix open reading frames were identified, four of which are, homologous to the components of toluene dioxygenase from Pseudomonas putida Fl and have been named bpha, bphe, bphf, and bphg. rom this comparison, biphenyl dioxygenase was found to be a multicomponent enzyme containing a two-subunit iron-sulfur protein, a ferredoxin, and a reductase. omparison of the large subunit of the iron-sulfur protein and the ferredoxin with other multicomponent dioxygenase identified amino acid sequences similar to Rieske iron-sulfur proteins for binding a [2Fe-2S] cluster. equences have also been identified in the reductase component that match the consensus sequence for FAD or NAD finding. ranscription of the biphenyl dioxygenase region was examined, and three transcription initiation sites were identified. ranscription initiating at the site furthest upstream is greatly increased when the LB400 cells are grown on biphenyl as the sole carbon source.

Record Details:

Record Type:DOCUMENT( REPORT )
Product Published Date:12/31/1992
Record Last Revised:12/22/2005
Record ID: 44727