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METABOLISM OF BENZO[A]PYRENE AND PERSISTENCE OF DNA ADDUCTS IN THE BROWN BULLHEAD (ICTALURUS NEBULOSUS)
Citation:
Sikka, H., A. Steward, C. Kandaswami, J. Rutkowski, J. Zaleski, S. Kumar, K. Earley, AND R. Gupta. METABOLISM OF BENZO[A]PYRENE AND PERSISTENCE OF DNA ADDUCTS IN THE BROWN BULLHEAD (ICTALURUS NEBULOSUS). U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-92/200 (NTIS PB92189067), 1991.
Description:
The in vitro metabolism of [3H]benzo[a]pyrene (BP) and [14C]benzo[a]pyrene-7,8-dihydrodiol (BP-7,8-diol) by liver of brown bullhead (Ictalurus Nebulosus) was characterized, as was the formation and persistence of BP-DNA adducts in vivo. ompared to rat liver microsomes, bullhead liver microsomes produced relatively larger amounts of BP-7,8-diol (predominantly the [-] enantiomer) and smaller amounts of BP-4,5-diol. P phase I metabolites were efficiently converted by freshly isolated bullhead hepatocytes to conjugates, predominantly glucuronides. P-7,8-dio was metabolized by hepatocytes 4-fold more rapidly than was BP and was converted to approximately equal amounts of glucuronides, glutathione conjugates and sulfates. P-DNA adducts formed in bullhead liver with a lag time of several days and maximum adduct formation at 25-30 days. he major adduct was anti-BPDE-deoxyguanosine.
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METABOLISM OF BENZO[A]PYRENE AND PERSISTENCE OF DNA ADDUCTS IN THE BROWN BULLHEAD (ICTALURUS NEBULOSUS)