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A CCL4/CHCL3 INTERACTION STUDY IN ISOLATED HEPATOCYTES: SELECTION OF A VEHICLE
Citation:
O'Hara, T., J. Borzelleca, E. Clarke, M. Sheppard, AND L. Condie. A CCL4/CHCL3 INTERACTION STUDY IN ISOLATED HEPATOCYTES: SELECTION OF A VEHICLE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-89/205 (NTIS PB90146192).
Description:
Emulphor, ethanol and DMS0 were evaluated as vehicles in studying the toxicity of CCl4 and CHCl3 in isolated hepatocytes. Appropriateness of the vehicle was determined by evaluating the following parameters: solubility of CCl4 and CHCl3 in the vehicle, cell injury (intracellular K+), cell death (LDH leakage) and lack of interaction (protection or enhanced toxicity) with CCl4 and CHCl3. The relative toxicity of the vehicles according to maximum no effect levels (v/v) was: emulphor (0.125%) > ethanol (1.0%) > DMSO (5.0%). Emulphor at toxic levels was inadequate to dissolve enough CCl4 to evaluate in this system. Ethanol (5.0, 2.5, 1.0, 0.5%) was more toxic than DMSO and interacted with both CCl4 and CHCl3 to enhance toxicity. DMSO (15.0, 5.0, 2.5%) did not significantly alter the toxicity of CCl4 and CHCl3; no interaction. These data suggest that DMSO should be the vehicle for evaluating the toxicity of CCl4 and CHCl3 and their mechanisms of action in the isolated hepatocyte.
