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GENOTOXIC ACTIVITY OF 1-CHLOROMETHYLPYRENE IN STOMACH EPITHELIUM IN VIVO: INSENSITIVITY OF THE SCINTILLATION UDS ASSAY
Citation:
Kennelly, J., M. Lane, J. Barker, G. Barber, H. Tinwell, J. Gallagher, B. Pool-Zobel, P. Schmezer, AND J. Ashby. GENOTOXIC ACTIVITY OF 1-CHLOROMETHYLPYRENE IN STOMACH EPITHELIUM IN VIVO: INSENSITIVITY OF THE SCINTILLATION UDS ASSAY. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-95/027.
Description:
An acknowledged weakness of current testing programs for genotoxic hazard has been the potential insensitivity of the established mouse bone ma,-row micronucleus test and rat liver UDS assays to direct-acting or short lived mutagens which may be consumed at the site of initial contact. n such cases in vivo test systems sampling tissues such as the skin or the stomach would provide valuable data. o test the hypothesis two stomach UDS assays were evaluated using the potent direct acting mutagen 1- chloromethypyrene (1-CMP). DS responses were not demonstrated for the potent direct acting 1-CMP and MNNG, the protease digestion/scintillation method for stomach UDS does not appear to have general value in a screening program for locally active genotoxic agents based on the high level of DNA adducts for example that we detected by 32P-postlabeling analysis in stomach DNA following gavage of 1-CMP.