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IMPORTANCE OF GLYCOLYSABLE SUBSTRATES FOR IN VITRO CAPACITATION OF HUMAN SPERMATOZOA
Citation:
Rogers, B. AND S. Perreault. IMPORTANCE OF GLYCOLYSABLE SUBSTRATES FOR IN VITRO CAPACITATION OF HUMAN SPERMATOZOA. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/358 (NTIS PB91163725).
Description:
To investigate the role of glycolysable substrate in capacitation of human spermatozoa, washed spermatozoa were incubated with or without sugars in BWW culture medium containing pyruvate and lactate. perm penetration into zona-free hamster eggs was then used as an indirect assay for capacitation. fter an 18 hour incubation, glucose (1 mg/ml) supported higher penetration of sperm into oocytes than either mannose or fructose, (60.7% vs. 28.2% or 21.5%, respectively) at the same concentration. enetration was even lower when medium contained the non-metabolizable sugar galactose (2.1% at 1 mg/ml). n the other hand, higher concentrations (5 or 10 mg/ml) of glucose, but not fructose, suppressed penetration, provided the glucose was present throughout the 18 hour incubation. hen caffeine, a stimulant of glycolysis in human super, was present along with glucose, capacitation was accelerated as evidenced by higher proportions of penetrated oocytes after 6 hours of sperm preincubation. his effect was not observed in glucose-free medium, however, where penetration remained low over a ten-hour incubation period. n these experiments, the percentage of motile sperm was unaffected by treatment, but the quality of motility was diminished in the absence of glucose. e conclude that stimulation of glycolysis may promote capacitation of human spermatozoa in vitro and that optimization of capacitation conditions is dependent upon both the type and concentration of glycolysable sugar present.