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EFFECTS OF INHALED PHOSGENE ON RAT LUNG ANTIOXIDANT SYSTEMS
Citation:
Jaskot, R., E. Grose, J. Richards, AND D. Doerfler. EFFECTS OF INHALED PHOSGENE ON RAT LUNG ANTIOXIDANT SYSTEMS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/395.
Description:
A concentration-response and CxT study were undertaken to determine the effect of phosgene (COCL2) inhalation to pulmonary antioxidant processes as determined by changes in endogenous glutathione (GSH) and antioxidant associated enzymes (GSH peroxidase, GSH reductase, glucose-6-phosphate dehydrogenase, and superoxide dismutase). ats were exposed to 0.0, 0.1, 0.25, 0.5, and 1.0 ppm phosgene for 4 hr. and 0.25 ppm phosgene for 8 hr. he endpoints were assayed at 0, 24, 48, 72 and 168 hr after exposure cessation. he lowest effective concentration was 0.1 ppm phosgene. t all concentrations, major effects were observed 24 to 48 hr after exposure, peaking at 48 to 72 hr postexposure and in some cases were still evident 168 hr after exposure. hese changes are similar to those observed for the oxidants 03 and NO2. lthough the suspected mechanism of initial damage between phosgene and these oxidants is different (acylation vs oxidation) the biological result is similar (i.e. damage, repair, and influx of cells), thus eliciting similar biochemical changes in response to pulmonary injury. hese changes, observed as increases in the pulmonary antioxidant system, appear to play a role in the lung's ability to recover from significant toxic injury to near normal pre-injury homeostasis.