Citation:

Matthews, J., M. Bianchini, R. Portier, AND e. al. DETERMINATION OF OPTIMAL TOXICANT LOADING FOR BIOLOGICAL CLOSURE OF A HAZARDOUS WASTE SITE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/D-89/163 (NTIS PB90116641).

Description:

Information on Phase I and Phase Il of a multitask effort to achieve biological closure of an abandoned hazardous waste site. aste materials, in the form of buried sludges and lagoon wastes, were examined. ptimal loading levels were evaluated on the basis of biodegradative potential tests and acute toxicity of leachate. icrobial ATP and microbial diversity were used in conjunction with Microtox tm tests to establish an acceptable land treatment experimental design. TP data indicated that both waste types stressed indigenous microbial populations through Day 10. owever, the microbial population showed recovery by Day 17. agoon wastes, applied at 4 and 8%, showed minimal stress and high ATP levels by Day 17. uried wastes showed comparable results for 2 to 4% waste loadings at Day 17. icrobial diversity indices confirmed ATP estimates, which suggested a maximum loading rate of 8% for lagoon wastes and 2 to 4% for buried wastes. C50 levels of leachates predicted 3.0 to 6% waste weight (wet basis) for buried waste. A loading rate of 4% for lagoon waste and 2.5% for buried waste was selected as the acceptable mean loading rate. C/MS data in subsequent Phase II studies documented significant biotransformation and biodegradation of the wastes at these optimized loading rates.

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