Biosyper Transcriptomic Assessment of Fetal and Neonatal Brain from Thyroid Hormone Disruption
Citation:
Gilbert, M., C. Wood, AND T. Stoker. Biosyper Transcriptomic Assessment of Fetal and Neonatal Brain from Thyroid Hormone Disruption. U.S. Environmental Protection Agency, Washington, DC, 2026. https://doi.org/10.23719/d-5h77
Impact/Purpose:
These data could be used to detail ontogeny of gene pathways from fetal to newborn brain in control animals and to identify pathways that are differentially affected by two distinct thyroid disrupting chemicals acting at different sites. The goal is to discover novel biomarkers of effect that could be used in assessing health hazards imposed by chemical-induced reductions in thyroid hormone during pregnancy.
Description:
Gene profiles in animals exposed to two prototypic thyroid hormone disrupting chemicals, one acting to inhibit thyroid hormone synthesis by inhibiting the enzyme thyroperoxidase (TPO), the other that blocks iodine uptake into the thyroid gland by inhibiting the sodium iodine symporter (NIS). Forebrain regions of fetal and newborn rats born to dams exposed to propylthiouracil or perchlorate were collected on gestational day 20, postnatal day 0, or postnatal day 2. Samples collected from offspring of dams exposed to the TPO inhibitor propylthiouracil or the NIS inhibitor perchlorate will be subjected a TempO-Seq® whole-genome targeted sequencing assay. This transcriptomic analysis in at these early ages will identify gene targets that may prove useful in detection and characterization of thyroid hormone dependent impairments in brain development and serve as biomarkers of effect. The goal is to discover novel biomarkers of effect that could be used in assessing health hazards imposed by chemical-induced reductions in thyroid hormone during pregnancy.
URLs/Downloads:
DOI: Biosyper Transcriptomic Assessment of Fetal and Neonatal Brain from Thyroid Hormone Disruption