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Reanalysis of PFO5DoA Levels in Blood from Wilmington, North Carolina, Residents, 2017-2018
Citation:
Kotlarz, N., J. McCord, N. Wiecha, R. Weed, M. Cuffney, J. Enders, M. Strynar, D. Knappe, B. Reich, AND J. Hoppin. Reanalysis of PFO5DoA Levels in Blood from Wilmington, North Carolina, Residents, 2017-2018. ENVIRONMENTAL HEALTH PERSPECTIVES. National Institute of Environmental Health Sciences (NIEHS), Research Triangle Park, NC, 132(2):27701, (2024). https://doi.org/10.1289/EHP13339
Impact/Purpose:
PFAS found previously in Wilmington NC residents exposed to contaminated drinking water were under reported in a prior publication. With new analytical methods and reanalysis modeled predictions of past measurements and new measurements for select PFAS are corrected in this manuscript.
Description:
The purpose of this letter is to correct previously reported blood serum concentrations for PFO5DoA in people in Wilmington, North Carolina (NC), in 2017-2018 [1]. Perfluoro-3,5,7,9,11-pentaoxadodecanoic acid (known as PFO5DoA; Chemical Abstracts Service Number [CASN] 39492-91-6, DTXSID50723994) is a perfluoroalkyl ether carboxylic acid (PFECA) containing six fully fluorinated carbon atoms. Our original analysis was done in 2018 using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). At the time, an authentic analytical standard for PFO5DoA was not commercially available and we were not aware of other laboratories analyzing human serum for PFO5DoA, which limited opportunities for interlaboratory comparisons. We have since discovered an unintended mass interference for calibration of our original PFO5DoA analysis that resulted in substantial underestimation of PFO5DoA concentrations in the samples. In 2022, we acquired an authentic analytical standard for PFO5DoA and re-analyzed a subset of the serum samples for PFO5DoA. We then used the PFO5DoA results from the re-analysis to develop a model to predict corrected PFO5DoA results for the remaining samples that were not re-analyzed. The detection frequency and median serum PFO5DoA concentration (99%, 9.2 ng/mL) in serum samples collected in Wilmington, NC, in 2017-2018, was higher than we previously reported (88%, 0.3 ng/mL). Additionally, we modified the analytical method to obtain measurements for 2,2,3,3-tetrafluoro-3-((1,1,1,2,3,3-hexafluoro-3-(1,2,2,2-tetrafluoro- ethoxy)propan-2-yl)oxy)propanoic acid (known as Hydro-EVE; CASN 773804-62-9; DTXSID60904459). Hydro-EVE is a polyfluorinated alkyl ether carboxylic acid which we had identified in some serum samples originally but could not quantify using our prior method.
URLs/Downloads:
DOI: Reanalysis of PFO5DoA Levels in Blood from Wilmington, North Carolina, Residents, 2017-2018
https://pubmed.ncbi.nlm.nih.gov/38306196/