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Rapid assessment bioaccumulation screening (RABS) for per-and polyfluoroalkyl substances in mice exposed to industrially impacted surface water.
Citation:
Bangma, J. Rapid assessment bioaccumulation screening (RABS) for per-and polyfluoroalkyl substances in mice exposed to industrially impacted surface water. SRP e-learning Webinar, Durham (Virtual), NC, November 08, 2023.
Impact/Purpose:
This presentation will present on the application of non-target analysis techniques and how they can be applied to mice treated with a mixture of novel PFAS derived from surface water. Results will reveal bio-accumulation potential of the various environmentally relevant PFAS a
Description:
The shift from PFOS and PFOA production in the past 20 years towards shorter chain and replacement PFAS has led to the environmental release of complex mixtures of emerging PFAS with unknown bioaccumulation potential and toxicology. Prioritizing research on merging PFAS in these complex mixtures is often limited by the lack of available chemical standards. We developed a study design that rapidly assesses which emerging PFAS in an environmentally derived mixture have the potential for mammalian bioaccumulation and can thereby prioritize these emerging chemicals for standard synthesis and individual toxicity testing. Surface water was collected at an impacted site adjacent to an industrial fluorochemical manufacturing facility and concentrated 100-fold via weak anion exchange, solid-phase extraction. The surface water extract contained dozens of emerging PFAS. Mice were dosed with the extract via oral gavage daily for 7 days, and tissues were collected approximately 24 hours after the last dose.
