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Appraisal of EPA qPCR method variations to reduce interferences by Great Lakes region water samples (abstract)
Citation:
Haugland, Rich AND S. Hertel. Appraisal of EPA qPCR method variations to reduce interferences by Great Lakes region water samples (abstract). State of Lake Michigan 2022, Traverse City, MI, October 24 - 26, 2022.
Impact/Purpose:
Inform public health officials and scientists on the current status of EPA efforts to support implementation of rapid recreational beach monitoring by quantitative PCR methods in Great Lakes region (EPA Region 5).
Description:
Work has proceeded to develop a more robust, but still rapid, sample preparation procedure that will mitigate occasional qPCR interference by Great Lakes region water samples. Results indicate that the addition of a proposed stabilizing material to the extraction buffer used in EPA qPCR methods is highly effective in mitigating losses of DNA recovery caused by a natural interfering substance in the environment (kaolinite clay). Optimal concentrations of the stabilizing material have been identified and a modified method incorporating this material is now being tested together with current EPA E. coli and enterococci qPCR methods on a variety of water samples to determine relative rates of interference and similarity of quantitative results.
