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Extracellular microRNA as Biomarkers of Environmental Chemical Health Hazard Identification
Citation:
Chorley, B. Extracellular microRNA as Biomarkers of Environmental Chemical Health Hazard Identification. Society of Toxicology 62nd Annual Meeting and ToxExpo, Nashville, TN, March 19 - 23, 2023. https://doi.org/10.23645/epacomptox.22755002
Impact/Purpose:
This is a presentation for a "Hot Topics" workshop at the 2023 Society of Toxicology Annual meeting. The presentation will cover mutiple research projects in our laboratory affliated with the development of miRNA biomarkers. This will highlight to the broader international toxicology community the utility of these biomarkers to indicate human helath adverse outcomes associated environmental chemical exposure.
Description:
MicroRNAs (miRNAs) are short, non-coding RNAs that serve to regulate the transcription and translation of target messenger RNA. MiRNAs can also be released by cells by both passive and active mechanisms in response to perturbation, including environmental toxicant exposure. The released miRNAs, which are stabilized by extracellular vesicles, protein, and lipids, can be measured in accessible matrices such as blood, urine, and sputum. Patterns of miRNA release may be tissue or mechanism-specific, therefore these measurements can be indicative of adaptation, stress, toxicity, and early disease or other adverse outcome development. In this talk, I will give current examples from our laboratory studies where we are developing these accessible miRNA biomarkers in different toxicological models, with focus on liver and kidney tissue. Examples will include chemical screening assay development efforts in vitro using media sampling, short-term rodent exposures to reference chemicals for specific modes-of-action, and utilization of these miRNA biomarkers to correlate to other markers of liver disease development in a residential human population with long-term exposure to pollutants. The goal of these studies is to augment existing toxicological measures with greater specificity, identify/characterize mechanistic linkages to adverse outcomes, and increase accessibility without invasive or destructive methods. This abstract does not necessarily reflect EPA policy.
URLs/Downloads:
DOI: Extracellular microRNA as Biomarkers of Environmental Chemical Health Hazard Identification
230307_SOT_HOTTOPIC_BNC V2.PDF (PDF, NA pp, 3215.638 KB, about PDF)