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Painting the Rainbow: Expanding high-throughput chemical hazard evaluation to ecotoxicology-relevant species with a rainbow trout gill cell line
Citation:
Harris, F., J. Nyffeler, C. Schaupp, S. Lasee, C. Willis, J. Nichols, B. Blackwell, K. Flynn, Dan Villeneuve, AND J. Harrill. Painting the Rainbow: Expanding high-throughput chemical hazard evaluation to ecotoxicology-relevant species with a rainbow trout gill cell line. 11th annual meeting of the American Society for Cellular and Computational Toxicology (ASCCT), Chapel Hill, NC, October 19 - 21, 2022. https://doi.org/10.23645/epacomptox.21732713
Impact/Purpose:
Presentation to the 11th annual meeting of the American Society for Cellular and Computational Toxicology (ASCCT) October 2022
Description:
Proposed high-throughput methods for chemical hazard evaluation at the United States Environmental Protection Agency emphasize the use of human-derived cells. To expand the applicability of these high-throughput methods to ecological hazard assessment, a previously developed high-throughput phenotypic profiling (HTPP) approach was applied to a rainbow trout gill cell line, RTgill-W1. In addition, the Organization for Economic Cooperation and Development’s test guideline for cell viability testing in RTgill-W1 cells (OECD TG249) was miniaturized to run in the same 384 well plate format and was performed in parallel with the HTPP assay. A HTPP screen was then conducted on a collection of 231 environmental chemicals of which 128 have existing in vivo rainbow trout toxicity data, 60 have been used in prior RTgill-W1 experiments, 110 have been tested in human cells with the HTPP assay, and 29 have been detected in the Great Lakes. Data from the HTPP screen was then used to estimate in vitro points-of-departure (PODs). Overall, 118 of 231 chemicals (51%) were active in the HTPP assay at concentrations below the threshold for cytotoxicity. The most potent chemicals included rotenone, several polyaromatic hydrocarbons (PAHs) and 6PPD-quinone, a degradation product of a chemical (6PPD) used in tire manufacturing. Further analysis will extrapolate in vitro PODs to water concentrations used in in vivo tests. These methods provide a cost-effective and efficient method for high-throughput hazard evaluation in an ecotoxicology-relevant cell model, informing a more complete hazard characterization for chemicals found in the environment. This abstract does not reflect US EPA policy.
URLs/Downloads:
DOI: Painting the Rainbow: Expanding high-throughput chemical hazard evaluation to ecotoxicology-relevant species with a rainbow trout gill cell line
FELIX HARRIS ASCCT_V8.PDF (PDF, NA pp, 3148.5 KB, about PDF)