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Development of Non-targeted Analysis Methods on GC-HRMS for Silicone Wristbands used as Passive Samplers
Citation:
Blum, D., M. Clifton, S. Newton, J. Sobus, AND E. Ulrich. Development of Non-targeted Analysis Methods on GC-HRMS for Silicone Wristbands used as Passive Samplers. SETAC NTA, Durham, NC, May 22 - 26, 2022. https://doi.org/10.23645/epacomptox.19775143
Impact/Purpose:
N/A
Description:
Rising industrial rates has caused concern regarding personal exposure and negative impacts on human health and the environment. Past atmospheric monitoring efforts have utilized stationary sampling systems which can be limited in number/location and could over or underestimate exposure to the population. Silicone wristbands have potential for capturing a wide range of chemicals to which humans are exposed. Targeted methods are limited by unique analytical methods and may be unable to identify unknowns. Non-target analysis (NTA) methods are beneficial for identifying emerging contaminants and more rapidly identifying known pollutants. NTA methods have been used to broaden the understanding of personal exposure by qualitatively identifying emerging contaminants and estimating concentrations. Gas chromatography (GC) methods can investigate broad swaths of chemical space and are somewhat orthogonal to more commonly used liquid chromatography (LC). A standardized and tested procedure analyzing silicone wristbands on GC-HRMS has not been established at EPA. In order to expand NTA in-house capabilities, method development was undertaken, with the intent to enable application of the method to the EPA’s Non-Targeted Analysis Collaborative Trial wristbands and real-world samples. Multiple methods for cleaning and extraction of the wristbands were tested for capture of the most analytes with the least amount of interference. Two cleaning methods were tested in conjunction with four extraction techniques. Isotopically labeled standards were used to evaluate recovery and instrument performance, and blanks were included to evaluate ubiquitous signals. Sample data was analyzed using Xcalibur and Compound Discover. The optimal cleaning method was chosen by lowest number of peaks produced, while the extraction method was chosen by highest number of peaks and best recovery rate of isotopically labeled standards that vary in mass and boiling point. This optimized procedure was applied to silicone wristbands deployed in various environments. Unknowns will be identified in accordance with Schymanski et al. Identification by NTA techniques using GC-HRMS data will allow greater confidence and breadth for identification of emerging contaminants and unknown species. This abstract does not necessarily reflect agency policy.
URLs/Downloads:
DOI: Development of Non-targeted Analysis Methods on GC-HRMS for Silicone Wristbands used as Passive Samplers
SETAC_NTA_DANIELLEBLUM2022 5-13-22.PDF (PDF, NA pp, 526.017 KB, about PDF)