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Use of Molecular Methods to Determine the Presence of Toxic Cyanobacteria in an Urban Lake - Cincinnati
Citation:
SantoDomingo, J. Use of Molecular Methods to Determine the Presence of Toxic Cyanobacteria in an Urban Lake - Cincinnati. Presented at Small Drinking Water Systems Webinar, Cincinnati, OH, April 26, 2022.
Impact/Purpose:
The presentation will provide examples of molecular methods used to monitor cyanobacterial blooms
Description:
This presentation introduces molecular monitoring approaches used in the detection and quantification of cyanobacterial groups and cyanotoxin genes implicated in harmful algal blooms. It focuses on results from next generation sequence analysis and qPCR/RT-qPCR methods used to characterize the cyanobacterial community structure, associated bacterial community, toxic cyanobacteria, and geographically localized genotypes (strains) or species. Those methods study cyanobacterial functional genes associated with nutrients (i.e., nitrogen cycle) in toxin production, their relationship to water quality parameters, and explore drivers of cyanotoxin production using mRNA-based sequence analysis. There is also discussion on the occurrence, distribution, and temporal-spatial variations of cyanobacteria, especially toxin-producers, and how to use as early warning systems for cyanotoxin production.