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Rapid assessment bioaccumulation screening (RABS) for per-and polyfluoroalkyl substances in mice exposed to industrially impacted surface water
Citation:
Bangma, J., T. Cantu, M. Strynar, A. Lindstrom, J. McCord, D. Hill, Chris Lau, N. Chernoff, AND J. Lang. Rapid assessment bioaccumulation screening (RABS) for per-and polyfluoroalkyl substances in mice exposed to industrially impacted surface water. FLUOROS Global 2021, Virtual, October 03 - 07, 2021.
Impact/Purpose:
The aim of this study was to determine if a rapid assessment in mice could predict which PFAS in a complex mixture have the potential for mammalian bioaccumulation and thus help to prioritize PFAS toxicity testing for complex industrial mixtures detected in drinking water.
Description:
The presence of µg/L concentrations of numerous per- and polyfluoroalkyl substances (PFAS) in the surface water has been reported in Bladen County, NC. The aim of this study was to determine if a rapid assessment in mice could predict which of these PFAS have the potential for mammalian bioaccumulation and thus help to prioritize PFAS toxicity testing. In April and May of 2018, water was collected in Bladen County downstream of an industrial outfall near the William O Huske Dam and concentrated by solid phase extraction to approximate 15-30 µg/mL of hexafluoropropylene oxide-dimer acid (HFPO-DA) in 90:10 water: ethanol. In addition, twelve other recently identified PFAS and five legacy PFAS were present in the dosing solution with concentrations ranging from 0.013 to 83 µg/mL. Balb-C mice were gavaged orally with the resulting concentrate once daily for seven days. Twenty-four hours after the last dose, liver, serum and urine were collected for analysis. Of the 13 emerging PFAS, polyfluorinated ether sulfonic acid byproduct-2 (PFESA-BP2), Hydro-EVE, PFO4DA and PFO5DoDA were most readily bioaccumulated in the serum and liver of both males and females, revealing similarities to recent publications highlighting bioaccumulation of the same PFAS in human serum in communities exposed to these contaminants via drinking water. This study design should facilitate prioritization for in vitro and in vivo toxicity testing of emerging alternative PFAS such as those newly identified in communities where complex industrial PFAS mixtures are detected in drinking water. (This abstract does not represent EPA policy)
