Citation:

Shanks, O., J. Hey, Mano Sivaganesan, J. Kralj, S. Servetas, M. Hunter, B. Toman, AND S. Jackson. Development and Performance of NIST SRM 2917 for Molecular Recreational Water Quality Testing. DHS/NIST Workshop: Standards to Support an Enduring Capability in Wastewater Surveillance for Public Health, Virtual, June 14 - 18, 2021.

Impact/Purpose:

EPA and other scientists have made significant strides in the development of advanced monitoring technologies for the characterization of fecal pollution.  Genetic technologies are now available that can provide same day notification of fecal pollution levels using enterococci and E. coli, as well as provide fecal pollution information for human, ruminant, canine, and avian sources in recreational water samples.  These methodologies rely on real-time quantitative PCR (qPCR) to estimate the concentration of genetic targets using a standard curve generated from a reference material.  National implementation of a qPCR-based technologies requires a high-quality reference material that is readily available to the public.  This project sought to develop and characterize the performance of a national standard reference material to function with 13 qPCR surface water quality testing methods in collaboration with the National Institute of Standards and Technology (NIST).  The resulting material, NIST SRM 2719 will help federal, state, and other local groups implement these advanced water quality testing technologies on a more consistent basis.

Description:

Product is an oral presentation describing a collaboration between EPA and the National Institute of Standards and Technology (NIST) to develop and characterize the performance of plasmid DNA Standard Reference Material (NIST SRM 2917) for the purpose of implementing 13 qPCR assays for recreational water quality monitoring applications.  Research supports SSWR 3.1.1 focusing on a high priority EPA Office of Water need described in the Five-Year Review of the 2012 Recreational Water Quality Criteria (EPA 823 18 001, May 2018).

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