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Integrating DNA-based biomonitoring into the United States National Aquatic Resource Surveys
Citation:
Mitchell, R., M. Bagley, AND J. Darling. Integrating DNA-based biomonitoring into the United States National Aquatic Resource Surveys. 2021 NALMS National Monitoring Conference, Virtual, April 19 - 23, 2021.
Impact/Purpose:
Talk at the National Monitoring Conference aimed at communicating needs of NARS for future incorporation of molecular biomonitoring indices
Description:
The National Aquatic Resource Surveys (NARS) program is a collaborative effort between the US Environmental Protection Agency, states, and tribes, aimed at assessing the status and trends of the nation’s lakes, rivers, wetlands, and coastal waters. The program employs a probabilistic survey design and standardized sampling and analytical protocols to generate scientifically valid, consistent national data. The rapid development of DNA-based biomonitoring has raised the possibility of integrating more informative and cost-effective methods for assessing biological endpoints as indicators of the ecological integrity of aquatic habitats. However, achieving this aim will require the establishment of vetted protocols ranging from field sampling, DNA preservation and extraction, and molecular and bioinformatics workflows in order to ensure that DNA data can be utilized alongside existing methods at a national scale. Here we elaborate on the specific requirements of the NARS program for acceptance of DNA-based ecological indicators. We discuss opportunities for incorporation of DNA-based approaches in the near term (e.g. applications involving periphyton or microbial endpoints) and outline criteria that must be met in order for these technologies to move from research to validated application. We further explore logistical challenges associated with standardization of collection and sample handling protocols conducted by diverse parties and we outline possible approaches to establishing minimum quality assurance standards that will prove robust in the face of rapidly evolving molecular technologies and analytical methods.
